【文獻(xiàn)標(biāo)題】Transcriptome analysis of the effect of pyrroloquinoline quinone disodium (PQQ·Na2) on reproductive performance in sows during gestation and lactation

【作者】Boru Zhang, Chenxi Wang, Wei Yang,et.al

【作者單位】東北農(nóng)業(yè)大學(xué)（ Northeast Agricultural University）

【文獻(xiàn)中引用產(chǎn)品】

牛免疫球蛋白G(IgG )ELISA試劑盒

牛免疫球蛋白A(IgA)ELISA試劑盒

牛免疫球蛋白M（IgM）ELISA試劑盒

【關(guān)鍵詞】Oxidative stress, Pyrroloquinoline quinone, Pyrroloquinoline quinone disodium, Reproductive performance,RNA-seq, Sow

【影響因子(IF)】5.8

【出版期刊】《Journal of Animal Science and Biotechnology》

【產(chǎn)品原文引用】

Evaluation of antioxidant enzyme activity and the concentration of nitric oxide

Superoxide dismutase (SOD), glutathione peroxidase(GSH-Px) and catalase (CAT) enzyme activities in the milk, plasma and placenta were determined using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) in accordance with our previous study. The results of the measurements were expressed as U/mL in plasma and milk and as U/mg protein in placenta. Lipid peroxidation in the plasma,milk and placenta was determined by measuring the amounts of malondialdehyde (MDA) through the thiobarbituric acid method using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing,China). The results of the measurements were expressed as nmol/mL in plasma and as nmol/mg protein in placenta. Nitric oxide (NO) and inducible NOS (iNOS) in the plasma and placenta of sows were determined using assay kits obtained from Jiancheng Biochemistry (Nanjing, China). The colostrum was analyzed for lactose,protein, fat, and total solids with a fully automatic milk analyzer (Milko ScanTM FT+ Analyzer, Foss). The milk samples were analyzed for immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) using immunoglobulin-specific kits (Jinma Biological Engineering Co., Ltd., Shanghai, China).

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